Difference between revisions of "Open Source Bioprinter"

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| Y-Carriage  || '''???''' || 1 || 30 € || [https://www.ebay.de/itm/Y-Carriage-Plate-for-Heated-Bed-Trespa-HPL-Reprap-3D-Printer-Prusa-i3/112031552702?hash=item1a1599d4be:g:oEoAAOSwrklVGaIC Ebay RepRap Universe]
 
| Y-Carriage  || '''???''' || 1 || 30 € || [https://www.ebay.de/itm/Y-Carriage-Plate-for-Heated-Bed-Trespa-HPL-Reprap-3D-Printer-Prusa-i3/112031552702?hash=item1a1599d4be:g:oEoAAOSwrklVGaIC Ebay RepRap Universe]
 
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| Threaded rod || E: '''???''' || 1 || '''???''' || '''Hamwa noch, oder?'''
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| Threaded rod || E: 160mm (d:8mm) || 1 || '''?''' || '''Hamwa noch, oder?'''
 
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| Smooth guiding rods (d: 8mm) || X: 360mm Y: 360mm Z: 320mm E: '''??''' || X & Y: x4 Z: x2 E: x2 || ~10€ (ebay) ~kA (proKilo) || [https://www.ebay.de/itm/V2A-Edelstahl-blank-1-4301-Vollmaterial-Lange-1000mm-Stab-Stabstahl-Rundstahl/192298732940?hash=item2cc5e5cd8c:m:mpANjWUpa90kt_FllSOFcyA ebay] / [https://www.prokilo.de/ ProKilo]
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| Smooth guiding rods (d: 8mm) || X: 360mm Y: 360mm Z: 320mm E: 2x205mm + 1x182mm || X & Y: x4 Z: x2 E: x3 || ~10€ (ebay) ~kA (proKilo) || [https://www.ebay.de/itm/V2A-Edelstahl-blank-1-4301-Vollmaterial-Lange-1000mm-Stab-Stabstahl-Rundstahl/192298732940?hash=item2cc5e5cd8c:m:mpANjWUpa90kt_FllSOFcyA ebay] / [https://www.prokilo.de/ ProKilo]
 
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| Linear Bearings || LM8UU + LM8LUU || Y&E(LM8LUU): x5 X&Z(LM8UU): x7 || Y: 15€ X&Z: 9€ || [https://www.ebay.de/itm/LM8LUU-Linearlager-lang-Kugellager-Bearing-Linear-Ball-Bush-CNC-3D-Drucker/162234170117?hash=item25c5e8fb05:g:iR0AAOSw3mpXDe2G LM8LUU rayntec ebay] [https://www.ebay.de/itm/LM8UU-Linearlager-Kugellager-Bearing-Linear-Ball-Bush-CNC-3D-Drucker/152102213037?hash=item2369ff95ad:g:F5UAAOxyVLNSp5IN LM8UU rayntec ebay]
 
| Linear Bearings || LM8UU + LM8LUU || Y&E(LM8LUU): x5 X&Z(LM8UU): x7 || Y: 15€ X&Z: 9€ || [https://www.ebay.de/itm/LM8LUU-Linearlager-lang-Kugellager-Bearing-Linear-Ball-Bush-CNC-3D-Drucker/162234170117?hash=item25c5e8fb05:g:iR0AAOSw3mpXDe2G LM8LUU rayntec ebay] [https://www.ebay.de/itm/LM8UU-Linearlager-Kugellager-Bearing-Linear-Ball-Bush-CNC-3D-Drucker/152102213037?hash=item2369ff95ad:g:F5UAAOxyVLNSp5IN LM8UU rayntec ebay]

Revision as of 15:56, 19 February 2018

The 'Nydus One Bioprinter' is an extrusion-based Bioprinter. It operates via a controllable mechanical press, to extrude cell-laden bioinks. Basis for the Bioprinter is a remixed version of the RepRap Prusa i3 MK2-X. The printer is capable of printing all three common cell sources: cell-lines, primary tissue and stem cells. To operate the Bioprinter, Dennis Ogiermann has written a "Composer". This open source pathplanning tool allows to dispense into standarized container and allows repetitive experiment designs. The 'Nydus One Bioprinter' was established using a experimental 'Microgel Approach'. Main idea of the approach is the use of a thermoreversible gelatin bath (called Slurry Bath) to print objects with complex geometry. The approach was published by Hinton et al. in 2015 (see Sources).


Development

To track and coordinate our development efforts we use an OpenProject repository: See OpenProject Homescreen


OSF Repository

To allow sharing the required soft- and hardware, we have created an OSF repository: Open Science Framework Bioprinter Project


Costs

Electronics

Component Details Amount Price Link
Motherboard mini-Rambo v1.3 Kit 1 120 $ Ultimachine Shop
Mechanical Endstop inlcuded 3 / see above
LCD Display 2004 LCD 1 10$ AliExpress
Heatbed Aluminium incl. Thermistor 1 45$ AliExpress
PINDA Probe Reddit 1 4$ AliExpress
NEMA17 Servo-Kit incl. threaded rod 3 + 2 110$ AliExpress
Pressure Sensor Interlink FSR 406 1 15$ Exp-Tech
Power Supply  ??? 1 17$ AliExpress
Thermistor HotEnd, req. 1 6€ reprapteile

Mechanics

Component Details Amount Price Link
Aluminium frame X: 360mm Y: 320mm Z: 330mm X: x3 Y: 2x Z: 2x 30€ Alu Profil Technik
Y-Carriage ??? 1 30 € Ebay RepRap Universe
Threaded rod E: 160mm (d:8mm) 1 ? Hamwa noch, oder?
Smooth guiding rods (d: 8mm) X: 360mm Y: 360mm Z: 320mm E: 2x205mm + 1x182mm X & Y: x4 Z: x2 E: x3 ~10€ (ebay) ~kA (proKilo) ebay / ProKilo
Linear Bearings LM8UU + LM8LUU Y&E(LM8LUU): x5 X&Z(LM8UU): x7 Y: 15€ X&Z: 9€ LM8LUU rayntec ebay LM8UU rayntec ebay
Toothed Belt 2 meter 1 2€ rayntec ebay
Pulley GT2-16 2 6€ rayntec ebay
Return Pulley ??? 2 8€ rayntec ebay

Parts (Screws etc.)

Component Details Amount Price Link
Winding Tube 10 meter 1 4$ AliExpress
Screws Frame&Axis Schraubengrößen in 50er Pacaks holen  ? Schraube&Mutter
Plastic Parts Frame&Axis Printed by Vitt ~30€ (white) Check OSF Repository & Sources
Powersupply Cable 2-adrige nachmessen am BP ??? Conrad

In summary: ~500-600€

Editable List


Sources

Basis

Frame Modifications

Assembly Guide

Extruder Parts

Microgel Approach