Difference between revisions of "Open Source Bioprinter"
Mustafasindi (talk | contribs) (→methods of bioprinting) |
(→Alginate Preparation) |
||
| Line 20: | Line 20: | ||
==== Alginate Preparation==== | ==== Alginate Preparation==== | ||
| + | |||
| + | |||
| + | \mathrm{CaCO_3 + 2 \ HCl \longrightarrow CaCl_2 + CO_2 + H_2O} | ||
== OSF Repository == | == OSF Repository == | ||
Revision as of 10:07, 1 March 2018
The 'Nydus One Bioprinter' is an extrusion-based Bioprinter. It operates via a controllable mechanical press, to extrude cell-laden bioinks. Basis for the Bioprinter is a remixed version of the RepRap Prusa i3 MK2-X. The printer is capable of printing all three common cell sources: cell-lines, primary tissue and stem cells. To operate the Bioprinter, Dennis Ogiermann has written a "Composer". This open source pathplanning tool allows to dispense into standarized container and allows repetitive experiment designs. The 'Nydus One Bioprinter' was established using a experimental 'Microgel Approach'. Main idea of the approach is the use of a thermoreversible gelatin bath (called Slurry Bath) to print objects with complex geometry. The approach was published by Hinton et al. in 2015 (see Sources).
Contents
Development
To track and coordinate our development efforts we use an OpenProject repository: See OpenProject Homescreen
Composer issues
- depending on object(geometry) to be printed and the print settings, some prints might not work (trial and error, may work with some settings, but we don't know them)
- the pathplanning seems to be off (Old Composer issue?)
- If you change the number of objects-to-be-printed
Methods of Bioprinting
Preparations
Microgel Preparation
Alginate Preparation
\mathrm{CaCO_3 + 2 \ HCl \longrightarrow CaCl_2 + CO_2 + H_2O}
OSF Repository
To allow sharing the required soft- and hardware, we have created an OSF repository: Open Science Framework Bioprinter Project
Costs
Electronics
| Component | Details | Amount | Price | Link |
| Motherboard | mini-Rambo v1.3 Kit | 1 | 120 $ | Ultimachine Shop |
| Mechanical Endstop | inlcuded | 3 | / | see above |
| LCD Display | 2004 LCD | 1 | 10$ | AliExpress |
| Heatbed | Aluminium incl. Thermistor | 1 | 45$ | AliExpress |
| PINDA Probe | 1 | 4$ | AliExpress | |
| NEMA17 | Servo-Kit incl. threaded rod | 3 + 2 | 110$ | AliExpress |
| Pressure Sensor | Interlink FSR 406 | 1 | 15$ | Exp-Tech |
| Power Supply | ??? | 1 | 17$ | AliExpress |
| Thermistor | HotEnd, req. | 1 | 6€ | reprapteile |
Mechanics
| Component | Details | Amount | Price | Link |
| Aluminium frame | X: 360mm Y: 320mm Z: 330mm | X: x3 Y: 2x Z: 2x | 30€ | Alu Profil Technik |
| Y-Carriage | ??? | 1 | 30 € | Ebay RepRap Universe |
| Threaded rod | E: 160mm (d:8mm) | 1 | ? | Hamwa noch, oder? |
| Smooth guiding rods (d: 8mm) | X: 360mm Y: 360mm Z: 320mm E: 2x205mm + 1x182mm | X & Y: x4 Z: x2 E: x3 | ~10€ (ebay) ~kA (proKilo) | ebay / ProKilo |
| Linear Bearings | LM8UU + LM8LUU | Y&E(LM8LUU): x5 X&Z(LM8UU): x7 | Y: 15€ X&Z: 9€ | LM8LUU rayntec ebay LM8UU rayntec ebay |
| Toothed Belt | 2 meter | 1 | 2€ | rayntec ebay |
| Pulley | GT2-16 | 2 | 6€ | rayntec ebay |
| Return Pulley | ??? | 2 | 8€ | rayntec ebay |
Parts (Screws etc.)
| Component | Details | Amount | Price | Link |
| Winding Tube | 10 meter | 1 | 4$ | AliExpress |
| Screws | Frame&Axis | Schraubengrößen in 50er Pacaks holen | ? | Schraube&Mutter |
| Plastic Parts | Frame&Axis | Printed by Vitt | ~30€ (white) | Check OSF Repository & Sources |
| Powersupply Cable | 2-adrige | nachmessen am BP | ??? | Conrad |
In summary: ~500-600€
