Open Source Bioprinter
The 'Nydus One Bioprinter' is an extrusion-based Bioprinter. It operates via a controllable mechanical press, to extrude cell-laden bioinks. Basis for the Bioprinter is a modified version of the RepRap Prusa i3 MK2-X. The printer is capable of printing all three common cell sources: cell-lines, primary tissue and stem cells. To operate the Bioprinter, Dennis Ogiermann has written a "Composer". This open source pathplanning tool allows to dispense into standarized containers and cell culture vessels and allows repetitive experiment designs. The 'Nydus One Bioprinter' was established using a experimental 'Microgel Approach'. Main idea of the approach is the use of a thermoreversible gelatin bath (called Slurry Bath) to print objects with complex geometry. The approach was published by Hinton et al. in 2015 (see Sources).
Contents
Development
To track and coordinate our development efforts for future Bioprinter iterations, we switched back to a special Trello board as our management of choice. Stay on track here: https://trello.com/b/vZAdSiBJ/bioprinter-sprint-1408-28082018
Bioprinting Software Bundle
CAD-Modeling: FreeCAD.
G-Code Generation: Slic3r.
Bioprinter Path Modification: Composer ( https://github.com/NydusOne/gcode-composer )
Composer issues
- depending on object(geometry) to be printed and the print settings, some prints might not work (trial and error, may work with some settings, but we don't know which settings are appropriate for what kind of structure)
- the pathplanning seems to be off (Old Composer issue, solved in the new one!)
- If you change the number of objects-to-be-printed later on, the composer will not recognize this and just print the initial number of constructs. To solve this issue, exit the composer and open again
Bioprinting Protocol
OSF Repository
To allow sharing the required soft- and hardware, we have created an OSF repository: Open Science Framework Bioprinter Project
Costs
Electronics
| Component | Details | Amount | Price | Link |
| Motherboard | mini-Rambo v1.3 Kit | 1 | 120 $ | Ultimachine Shop |
| Mechanical Endstop | inlcuded | 3 | / | see above |
| LCD Display | 2004 LCD | 1 | 10$ | AliExpress |
| Heatbed | Aluminium incl. Thermistor | 1 | 45$ | AliExpress |
| PINDA Probe | 1 | 4$ | AliExpress | |
| NEMA17 | Servo-Kit incl. threaded rod | 3 + 2 | 110$ | AliExpress |
| Pressure Sensor | Interlink FSR 406 | 1 | 15$ | Exp-Tech |
| Power Supply | ??? | 1 | 17$ | AliExpress |
| Thermistor | HotEnd, req. | 1 | 6€ | reprapteile |
Mechanics
| Component | Details | Amount | Price | Link |
| Aluminium frame | X: 360mm Y: 320mm Z: 330mm | X: x3 Y: 2x Z: 2x | 30€ | Alu Profil Technik |
| Y-Carriage | ??? | 1 | 30 € | Ebay RepRap Universe |
| Threaded rod | E: 160mm (d:8mm) | 1 | ? | Hamwa noch, oder? |
| Smooth guiding rods (d: 8mm) | X: 360mm Y: 360mm Z: 320mm E: 2x205mm + 1x182mm | X & Y: x4 Z: x2 E: x3 | ~10€ (ebay) ~kA (proKilo) | ebay / ProKilo |
| Linear Bearings | LM8UU + LM8LUU | Y&E(LM8LUU): x5 X&Z(LM8UU): x7 | Y: 15€ X&Z: 9€ | LM8LUU rayntec ebay LM8UU rayntec ebay |
| Toothed Belt | 2 meter | 1 | 2€ | rayntec ebay |
| Pulley | GT2-16/623h bearing housing | 2 | 6€ | rayntec ebay |
| Return Pulley | 623h bearing housing | 2 | 8€ | rayntec ebay |
Parts (Screws etc.)
| Component | Details | Amount | Price | Link |
| Winding Tube | 10 meter | 1 | 4$ | AliExpress |
| Screws | Frame&Axis | Schraubengrößen in 50er Pacaks holen | ? | Schraube&Mutter |
| Plastic Parts | Frame&Axis | Printed by Vitt | ~30€ (white) | Check OSF Repository & Sources |
| Powersupply Cable | 2-adrige | nachmessen am BP | ??? | Conrad |
In summary: ~500-600€
